Restriction endonucleases are useful in gene cloning because:
A) they can cut DNA at specific sequences.
B) they can join DNA molecules that have been cut at specific sequences.
C) they all make cohesive ends when they cut DNA.
D) they all make 5' phosphate extensions and recessed 3' OH groups that can be rejoined by DNA ligase.
E) they can both cut and rejoin DNA molecules.

Question

Quizplus · Accepted Answer

Restriction endonucleases are enzymes that cut DNA at specific recognition sequences. By using different endonucleases, researchers can cut DNA at specific locations and thus manipulate the DNA for gene cloning purposes.

Restriction Endonucleases Are Useful in Gene Cloning Because