Which of the following statements about translation elongation in bacteria is TRUE?
A) Charged amino acids are escorted to the A site of the ribosome by an initiation factor.
B) The growing polypeptide chain is moved from the A site to the P site before peptide bond formation.
C) GTP hydrolysis occurs after moving the aminoacyl-tRNA into the A site and before peptide bond formation.
D) The entire peptidyl-tRNA is translocated from the P site to the A site during peptide bond formation.
E) The uncharged tRNA leaves from the E site as the ribosome moves one codon in the 5' direction.

Question

Quizplus · Accepted Answer

GTP is hydrolyzed by the elongation factor EF-Tu after moving the aminoacyl-tRNA into the A site and before peptide bond formation. The correct sequence of events during translation elongation in bacteria is: 1) The charged aminoacyl-tRNA binds to the A site with the help of EF-Tu and GTP. 2) The ribosome catalyzes peptide bond formation between the amino acid in the P site and the aminoacyl-tRNA in the A site. 3) GTP is hydrolyzed by EF-Tu and the uncharged tRNA is released from the E site. 4) The ribosome translocates by one codon in the 5' direction, moving the peptidyl-tRNA from the A site to the P site and creating an empty A site for the next aminoacyl-tRNA. 5) EF-G (elongation factor G) promotes ribosome translocation and requires GTP hydrolysis.

Which of the Following Statements About Translation Elongation in Bacteria