Which technique could be used for molecular weight (mass)determinations in proteins?
A)X- ray diffraction
B)chromatofocusing
C)diethylaminoethyl (DEAE)cellulose chromatography
D)affinity chromatography
E)SDS-PAGE

Question

Quizplus · Accepted Answer

SDS-PAGE (polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate) is a commonly used technique for determining the molecular weight of proteins. The technique separates proteins based on their size, with smaller proteins migrating more quickly through the gel than larger ones. When combined with a protein ladder of known sizes, the molecular weight of a protein can be estimated based on its migration distance relative to the ladder.

Which Technique Could Be Used for Molecular Weight (Mass)determinations in Proteins