Why do phenol and buffered saline separate into two phases when one centrifuges the mixture during the DNA extraction procedure?
A)They are soluble.
B)They are miscible.
C)They are immiscible.
D)One freezes and the other does not.
E)They are both hydrophobic.

Question

Quizplus · Accepted Answer

Phenol and buffered saline are immiscible, which means they cannot mix completely to form a homogeneous solution. This leads to the formation of two separate phases when centrifuged, with phenol forming the upper phase and buffered saline forming the lower phase.

Why Do Phenol and Buffered Saline Separate into Two Phases