How are fragments already in a mass spectrometer further fragmented if this becomes necessary?
A)injection of pepsin
B)injection of more trypsin
C)collision of peptides with an inert gas
D)collision of peptides with nitrogen gas
E)collision of peptides with oxygen

Question

Quizplus · Accepted Answer

Fragments in a mass spectrometer can be further fragmented through collision with an inert gas, such as argon or helium. This process, known as collision-induced dissociation (CID) or collision-induced fragmentation (CIF), can break apart larger peptides into smaller fragments for analysis. Alternatively, pepsin and trypsin are proteolytic enzymes used for protein digestion and are not typically used to further fragment peptides in a mass spectrometer. Nitrogen and oxygen gases are not commonly used for fragmentation in mass spectrometry.

How Are Fragments Already in a Mass Spectrometer Further Fragmented