Which of the following is a feature of "third generation" sequencers?
A)Sequencing of single molecules without amplification
B)Generation of long reads of 1000 nucleotides or more
C)Laser-based detection of nucleotide incorporation
D)Identification of single nucleotides passing through nanopores
E)All of the above

Question

Quizplus · Accepted Answer

All of the options listed are features of "third generation" sequencers. These technologies include PacBio, Oxford Nanopore, and Ion Torrent. PacBio sequencing involves the real-time detection of polymerase activity using laser-based detection of nucleotide incorporation, which allows the sequencing of single molecules without amplification. Oxford Nanopore and Ion Torrent use nanopore technology to identify single nucleotides passing through the pore, generating long reads of 1000 nucleotides or more. Therefore, all of the options are correct answers.

Which of the Following Is a Feature of "Third Generation