What enzyme is used to replace the RNA of a DNA-RNA hybrid with DNA during the preparation of a cDNA library?
A)RNase H
B)reverse transcriptase
C)DNA polymerase I
D)RNase K
E)DNA ligase

Question

Quizplus · Accepted Answer

DNA polymerase I is used to replace the RNA strand of a DNA-RNA hybrid with DNA during the preparation of a cDNA library. It has both 5' to 3' polymerase activity and 5' to 3' exonuclease activity, which allows it to remove RNA primers and replace them with DNA.

What Enzyme Is Used to Replace the RNA of a DNA-RNA