Question 1

After exonucleases have excised the damaged portion of DNA,how can DNA polymerase fill in the gap? Is there a primer?

Accepted Answer

The answer of After exonucleases have excised the damaged portion...

Question 2

The DNA polymerases in a relatively large family of polymerases in which each member is specialized for incorporating nucleotides opposite particular types of DNA lesions in the template strand engage in a type of DNA synthesis called __________.They have an unusually spacious active site that ____________.

Accepted Answer

The given description matches the process of translesion synthesis, which is performed by specialized DNA polymerases that are able to incorporate nucleotides opposite certain kinds of DNA lesions. These polymerases have a more spacious active site that can accommodate altered nucleotides that do not fit in the active site of normal DNA polymerases used in replication. Hence, option B is the correct choice.

Question 3

You are the enzyme responsible for carrying out mismatch repair.You notice a mismatched pair in the DNA strand and you know it is your job to remove one of the bases in the mismatched pair.How would you decide which nucleotide to remove?

Accepted Answer

The answer of You are the enzyme responsible for carrying...

Question 4

What enzyme activity are the Mcm proteins (Mcm2 - Mcm7)thought to exhibit once they have been loaded onto the replication origin and associated into a ring-shaped complex? What role does this enzyme activity play in replication and to what protein in E.coli is it analogous?

Accepted Answer

The answer of What enzyme activity are the Mcm proteins...

Question 5

You are studying replication in a particular bacterial strain.At 37°C,the bacteria appear perfectly normal.If the temperature is raised to 43°C,however,they stop growing and are unable to replicate their DNA.If some of them are placed back at 37°C,they begin to replicate their DNA and are again perfectly normal.What is wrong?

Accepted Answer

The answer of You are studying replication in a particular...

Question 6

What happens once damage caused by lesions in the genome,like a thymidine dimer,is bypassed during replication and replication must continue?

Accepted Answer

Once the damage is bypassed, the normal replicative DNA polymerase resumes replication.

Quiz 13: Dna Replication and Repair

After exonucleases have excised the damaged portion of DNA,how can DNA polymerase fill in the gap? Is there a primer?

The DNA polymerases in a relatively large family of polymerases in which each member is specialized for incorporating nucleotides opposite particular types of DNA lesions in the template strand engage in a type of DNA synthesis called .They have an unusually spacious active site that __.

You are the enzyme responsible for carrying out mismatch repair.You notice a mismatched pair in the DNA strand and you know it is your job to remove one of the bases in the mismatched pair.How would you decide which nucleotide to remove?

What enzyme activity are the Mcm proteins (Mcm2 - Mcm7)thought to exhibit once they have been loaded onto the replication origin and associated into a ring-shaped complex? What role does this enzyme activity play in replication and to what protein in E.coli is it analogous?

What happens once damage caused by lesions in the genome,like a thymidine dimer,is bypassed during replication and replication must continue?