Why does centrifugation at high speeds proceed in a near vacuum?
A)to maximize frictional resistance to the rotor's motion
B)to minimize frictional resistance to the rotor's motion
C)to balance the rotor
D)to stabilize the rotor
E)to minimize pH fluctuations

Question

Quizplus · Accepted Answer

Centrifugation at high speeds can generate a significant amount of heat due to frictional resistance between the rotor and the surrounding air molecules. This can lead to denaturation or degradation of sensitive biomolecules being separated. Therefore, reducing the frictional resistance by carrying out the procedure in a near vacuum can minimize these effects and improve the accuracy of the separation. Additionally, a near vacuum can reduce the air drag force acting on the rotor, allowing it to spin at higher speeds without becoming unstable or unbalanced.

Why Does Centrifugation at High Speeds Proceed in a near Vacuum