Unwinding prior to ligation results in:
A) relaxed DNA.
B) a wider major groove.
C) negative supercoiling.
D) positive supercoiling.
E) None of the above.

Question

Quizplus · Accepted Answer

Unwinding DNA prior to ligation introduces negative supercoiling because it involves the underwinding or twisting in the opposite direction of the helix's natural twist, leading to a strain that manifests as negative supercoiling.

Unwinding Prior to Ligation Results In