When [S]

Question

When [S] << K_M, the enzymatic velocity depends on: A) the values of k_cat/K_M, [S], and [E]t. B) the V_maxof the reaction. C) the affinity of the substrate for the catalytic site. D) k_cat. E) the formation of the ES complex.

Quizplus · Accepted Answer

When [S] is much less than K_M, the enzymatic velocity is in the first-order kinetics regime, depending primarily on the concentration of substrate [S], the total enzyme concentration [E]t, and the efficiency of the enzyme, which can be represented by the term kcat/KM. This reflects the enzyme's catalytic efficiency in converting substrate to product under these conditions.

When [S] << KM, the Enzymatic Velocity Depends On

When [S] << K_M, the Enzymatic Velocity Depends On