You are setting up a PCR reaction and add a primer for one end of the target sequence, but you forget to add the primer for the other end of the target sequence. If you added the other necessary components, what do you expect to observe after running the PCR reaction for 40 cycles?
A) The PCR reaction will produce billions of copies of the target DNA.
B) The PCR reaction will produce millions of copies of the target DNA.
C) The PCR reaction will produce one copy of the target DNA.
D) The PCR reaction will produce zero copies of the target DNA.

Question

Quizplus · Accepted Answer

Both primers are required for the PCR reaction to occur. Without the primer for the other end of the target sequence, the reaction will not occur and produce zero copies of the target DNA.

You Are Setting Up a PCR Reaction and Add a Primer