Suppose a researcher is interested in using the CRISPR-Cas9 system to knock out the phosphofructokinase gene of a rat skin cell that codes for an enzyme that is critical for glycolysis to occur. The researcher added the Cas9 enzyme to the cell but forgot to add the guide RNA. What result do you think the researcher will observe?
A) Glycolysis will proceed normally in the cell.
B) The phosphofructokinase enzyme will be expressed but will have lower activity.
C) Cas9 will specifically cut the phosphofructokinase gene.
D) There will be mutations in the phosphofructokinase gene.

Question

Quizplus · Accepted Answer

Without the guide RNA, the Cas9 enzyme will not be able to target and cut the specific phosphofructokinase gene, and therefore the gene will remain intact. This means that the cell will still be able to produce the enzyme and glycolysis will proceed normally.

Suppose a Researcher Is Interested in Using the CRISPR-Cas9 System