What technique can be used to determine DNA-protein interactions?
A) Western blotting
B) PCR
C) DNAse I foot printing
D) restriction enzyme analysis

Question

Quizplus · Accepted Answer

DNAse I foot printing is the best technique to determine DNA-protein interactions because it involves digesting the DNA with DNAse I in the presence of the protein of interest, which protects certain regions of the DNA from digestion. This results in a pattern of fragments that can be visualized by gel electrophoresis, revealing the specific regions of the DNA that are bound by the protein. Western blotting and PCR are not suitable for this purpose, as they do not directly measure protein-DNA interactions. Restriction enzyme analysis may be useful for studying DNA sequence variations, but it does not provide information about protein binding.

What Technique Can Be Used to Determine DNA-Protein Interactions