You are using CRISPR-Cas technology to introduce a single nucleotide change in a gene of interest in living cells. You are designing your experiment. In which component of the reaction will you engineer the single nucleotide change?
A) Donor DNA
B) Spacer RNA
C) Repeat RNA
D) Linker RNA
E) tracrRNA

Question

Quizplus · Accepted Answer

The donor DNA is the component that contains the desired nucleotide change and is used to replace the existing sequence in the gene of interest during CRISPR-Cas editing. Therefore, engineering the single nucleotide change into the donor DNA will be the most effective way to introduce the desired change into the living cells.

You Are Using CRISPR-Cas Technology to Introduce a Single Nucleotide