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A Fragment of DNA Is Cloned into a Plasmid with a Sequencing

Question 39

Multiple Choice

A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site.After dideoxy sequencing, the gel pattern shown in this diagram is obtained.What was the sequence of the DNA strand that acted as the template in the sequencing reaction? A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site.After dideoxy sequencing, the gel pattern shown in this diagram is obtained.What was the sequence of the DNA strand that acted as the template in the sequencing reaction?   A) 5' GCTAGCA 3' B) 5' ACGATCG 3' C) 5' TGCTAGC 3' D) 5' CGATCGT 3' E) None of the answers is correct.


A) 5' GCTAGCA 3'
B) 5' ACGATCG 3'
C) 5' TGCTAGC 3'
D) 5' CGATCGT 3'
E) None of the answers is correct.

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