Question 1

If you were asked to isolate total RNA from two unknown samples and then were required to identify if the RNA was from prokaryotes or eukaryotes, what aspects regarding the classes of RNA present would help you distinguish one from the other?

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Question 2

What would you add to an in vitro transcription system that contains an E.coli gene in order to get transcription that begins from the normal transcription start site?

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Question 3

What is an snRNP and what role does it play in the cell?

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Question 4

Discuss the features of C.elegans that makes it an important model system for studies of animal genetics and development.Include in your answer some unique features of C.elegans compared to other model systems.

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Question 5

Compare rho-dependent and rho-independent transcription termination in terms of their use of RNA secondary structures.

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Question 6

In your own words, list a comprehensive definition for "gene" at the molecular level.

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Question 7

The discovery of ribozymes led to the theory that the evolution of life on Earth began with an "RNA world." Describe the chemical properties and functions of RNA that would allow it to be the basis of the first self-replicating systems.

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Question 8

What are the three different RNA polymerases found in all eukaryotes, and what types of genes do they transcribe?

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Question 9

For a time, a gene was commonly considered to be a sequence of DNA that encodes a polypeptide.Critique this outdated notion of what a gene is and propose a more comprehensive definition.

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Question 10

Which of the following small RNA types is unique to prokaryotes?

Accepted Answer

crRNA (CRISPR RNA) is a unique small RNA found only in prokaryotes, specifically in the CRISPR-Cas system, which helps in defense against viral or plasmid DNA. A, C, D, and E are all small RNA types found in eukaryotes. siRNA (small interfering RNA) and miRNA (microRNA) are involved in gene regulation and silencing, piRNA (PIWI-interacting RNA) plays a role in silencing transposons during germ cell development, and lncRNA (long non-coding RNA) is involved in various regulatory functions.

Quiz 10: From DNA to Proteins- Transcription and Rna Processing