Which of the following vectors would be most efficient to use to amplify fragments ~300 kb in length?
A)BACs
B)plasmids
C)YACs
D)$\lambda$ phage
E)cosmids

Question

Quizplus · Accepted Answer

YACs (yeast artificial chromosomes) are the most efficient vectors to use for amplifying large fragments, such as ones that are ~300 kb in length. YACs are capable of holding inserts up to 1 Mb, making them ideal for larger genomic regions. BACs (bacterial artificial chromosomes), plasmids, and cosmids are all limited in their insert size, with BACs being the largest at ~200 kb. F1F1F1S1F1F1F10 phage is not used for cloning large fragments.

Which of the Following Vectors Would Be Most Efficient to Use