If you want to express two different genes on different plasmids in a bacterial host, which procedure will NOT be successful?
A) using plasmids that have the same promoter sequence
B) using EcoRI and BamHI restriction endonucleases to clone the genes into their respective plasmids
C) placing different fusion tags on each of the genes when cloning them
D) using plasmids with the same origin of replication
E) using plasmids that have different selectable markers

Question

Quizplus · Accepted Answer

Using plasmids with the same origin of replication in the same bacterial host can lead to issues with plasmid stability and maintenance, as they compete for the same replication machinery. This can result in the loss of one or both plasmids from the host cells, making the procedure unsuccessful for expressing two different genes.

If You Want to Express Two Different Genes on Different