Match Each Feature of the Plasmid PBR322 (At Left) with One

Question 65

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Match each feature of the plasmid pBR322 (at left) with one appropriate description presented (at right) (see illustration of pBR322 below). Descriptions may be used more than once. Match each feature of the plasmid pBR322 (at left) with one appropriate description presented (at right) (see illustration of pBR322 below). Descriptions may be used more than once.   ____ amp<sup>R</sup> sequence (a) Permits selection of bacteria containing the plasmid. ____ ori sequence (b) A sequence required for packaging recombinant plasmids ____ tet<sup>R</sup> into bacteriophage. ____ BamHI sequence (c) Origin of replication. ____ PstI sequence (d) Cleavage of the plasmid here does not affect antibiotic sequence resistance genes. (e) Insertion of foreign DNA here permits identification of bacteria containing recombinant plasmids . ____ ampR sequence
(a) Permits selection of bacteria containing the plasmid.
____ ori sequence
(b) A sequence required for packaging recombinant plasmids
____ tetR
into bacteriophage.
____ BamHI sequence
(c) Origin of replication.
____ PstI sequence
(d) Cleavage of the plasmid here does not affect antibiotic
sequence resistance genes.
(e) Insertion of foreign DNA here permits identification of
bacteria containing recombinant plasmids .

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