If you wanted to study the effects of inactivating a specific gene on development of a mouse, which technique or system would you use to inactivate that gene?
A) transfection
B) CRISPR/Cas9
C) tandem affinity purification
D) yeast two-hybrid
E) oligonucleotide-directed mutagenesis

Question

Quizplus · Accepted Answer

CRISPR/Cas9 is a genome-editing system that can specifically target and inactivate a specific gene in a mouse. The technique has high efficiency and specificity, making it a powerful tool for gene editing. Transfection, tandem affinity purification, yeast two-hybrid, and oligonucleotide-directed mutagenesis are not ideal techniques for inactivating a specific gene in a mouse.

If You Wanted to Study the Effects of Inactivating a Specific