If you want to determine which proteins might be interacting in a cell, which technique would you use to examine the interaction in a living cell?
A) yeast two-hybrid analysis
B) immunoprecipitation
C) a DNA microarray
D) immunofluorescence
E) comparative genomics

Question

Quizplus · Accepted Answer

Yeast two-hybrid analysis is a technique used to identify protein-protein interactions in living cells. In this method, two proteins are fused to different domains of a transcription factor. If these two proteins interact in the cell, the transcription factor is reconstituted and activates the expression of a reporter gene, allowing for easy detection of the interaction. This technique has been widely used for studying protein-protein interactions in various organisms, including yeast and mammalian cells.

If You Want to Determine Which Proteins Might Be Interacting