According to the sequence below (note that the DNA is written in the 5' $\rightarrow$ 3' direction, and the gene of interest is underlined), the BEST reverse primer to use for this gene is:
A) 5' ATTGAATCCTTGGCT GGTGA 3'.
B) ' ATTGAATCCTTGGCT GGTGA 5'.
C) 5' TCACCAGCCAAGGATTCAAT 3'.
D) 3' TCACCAGCCAAGGATTCAAT 5''.
E) 3' GGTGAGTCGG TTCAATTCGT 5'.

Question

According to the sequence below (note that the DNA is written in the 5' $\rightarrow$ 3' direction, and the gene of interest is underlined), the BEST reverse primer to use for this gene is: 
A) 5' ATTGAATCCTTGGCT GGTGA 3'. 
B) ' ATTGAATCCTTGGCT GGTGA 5'. 
C) 5' TCACCAGCCAAGGATTCAAT 3'. 
D) 3' TCACCAGCCAAGGATTCAAT 5''. 
E) 3' GGTGAGTCGG TTCAATTCGT 5'.

Quizplus · Accepted Answer

The reverse primer should start at or near the end of the target sequence and proceed towards the start of the sequence. In this case, the gene of interest is underlined and the reverse primer should start at the 3' end of the sequence. Option C starts at the correct position and has a length of 20 nucleotides, which is a suitable length for a reverse primer. The other options either start too far from the end of the target sequence (options A and E) or start at the 5' end of the sequence (options B and D).

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