A next-generation method of DNA sequencing, 454 sequencing, uses pyrosequencing in which the addition of nucleotides is detected by a:
A) radioactive signal.
B) fluorescently labeled dNTP.
C) pulse from a laser.
D) flash of light.
E) nano-ruler's physical movement of 4.54 nm detected by laser.

Question

Quizplus · Accepted Answer

Pyrosequencing in 454 sequencing uses a method where the addition of nucleotides is detected through the release of a flash of light. This is done by incorporating enzymes that generate light emission as each nucleotide is incorporated into the growing DNA strand.

A Next-Generation Method of DNA Sequencing, 454 Sequencing, Uses Pyrosequencing