To determine the isoelectric point of a protein, first establish that a gel:

Question

Quizplus · Accepted Answer

The isoelectric point of a protein is the pH at which it carries no net electrical charge. To determine this point, a gel with a stable pH gradient is needed, which can be achieved by distributing ampholytes (molecules with both acidic and basic groups) in an electric field. As the protein moves through the pH gradient, it will eventually reach a point where its net charge is zero, indicating the isoelectric point. The other options (A, C, D, and E) do not directly relate to determining the isoelectric point of a protein.

To Determine the Isoelectric Point of a Protein, First Establish