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One Method Used to Prevent Disulfide Bond Interference with Protein

Question 34

Multiple Choice

One method used to prevent disulfide bond interference with protein sequencing procedures is:


A) cleaving proteins with proteases that specifically recognize disulfide bonds.
B) protecting the disulfide bridge against spontaneous reduction to cysteinyl sulfhydryl groups.
C) reducing disulfide bridges and preventing their re-formation by further modifying the -SH groups.
D) removing cystines from protein sequences by proteolytic cleavage.
E) sequencing proteins that do not contain cysteinyl residues.

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