By increasing the spacing between sequence 1 and sequence 2 in the leader peptide of the trp operon of E. coli, attenuation compared with the normal sequence is:

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Increasing the spacing between sequence 1 and sequence 2 in the leader peptide of the trp operon in E. coli disrupts the formation of the antiterminator structure (2-3 loop), favoring the formation of the terminator structure (3-4 loop) and leading to premature termination of transcription. This results in decreased attenuation efficiency, as the RNA polymerase is more likely to disengage before transcribing the operon's structural genes, especially under conditions where tryptophan is not abundant.

By Increasing the Spacing Between Sequence 1 and Sequence 2