The 5' $\rightarrow$3' exonuclease activity of E. coli DNA polymerase I is involved in:

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The 5' F1F1F1S1F1F1F10 3' exonuclease activity of E. coli DNA polymerase I is involved in removal of RNA primers by nick translation. This activity is essentially involved in removing the RNA primers that were synthesized by primase and replacing it with DNA nucleotides by the polymerase activity of DNA polymerase I in the lagging strand during DNA replication. Once the RNA primer is removed by the exonuclease activity, DNA polymerase I initiates filling the gap by adding nucleotides in a 5'→3' direction, also known as nick translation, and the gap is sealed by DNA ligase.

The 5 →\rightarrow→ 3' Exonuclease Activity of E

The 5 $\rightarrow$ 3' Exonuclease Activity of E