During the reaction of pyruvate carboxylase, CO₂ is NOT covalently attached to:

Question

Quizplus · Accepted Answer

Pyruvate carboxylase catalyzes the ATP-dependent carboxylation of pyruvate to form oxaloacetate, utilizing a biotin prosthetic group. During the reaction, a carboxybiotin intermediate is formed as CO₂ is covalently attached to a specific lysine residue on the enzyme. Therefore, the correct answer is D because lysine is the amino acid that CO₂ is covalently attached to during the reaction, while phosphate and pyruvate are involved in the reaction but not covalently attached to CO₂.

During the Reaction of Pyruvate Carboxylase, CO2 Is NOT Covalently

During the Reaction of Pyruvate Carboxylase, CO₂ Is NOT Covalently