Cells must be lysed to release the protein of interest if ________.

Name: Cells must be lysed to release the protein of interest if ________.
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Question

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Intracellular proteins are located within the cell and cannot be obtained without lysing the cell to release the protein. Proteins that are secreted into the media would not require cell lysis as they are already in the media. The hydrophobic or hydrophilic nature of the protein or its charge would not necessarily determine whether cell lysis is needed.

Cells Must Be Lysed to Release the Protein of Interest