Rattlesnakes Contract Their Tail Shaker Muscles at Frequencies Up to 90

Rattlesnakes contract their tail shaker muscles at frequencies up to 90 hertz (90 times a second) to create their rattling sound. You compare the contraction frequency of rattlesnake tail muscles at 20°C and 35°C. Do you expect to see an effect of temperature on contraction frequency in this experiment? Why or why not?

A) no, because protein structure is not affected by temperature
B) no, because neither of these temperatures is seen in the natural environment of a rattlesnake
C) maybe, it would depend on whether the snakes are male or female
D) yes, because muscle contraction is an enzymatic process, and thus will be temperature dependent
E) yes, because all biological structures show changes when the temperature changes from 20°C to 35°C