In order for a nucleotide to be added to a growing RNA strand, the polymerase ensures that hydrogen bonds between the incoming base and the template strand are properly made before the high-energy phosphate bond is cleaved.

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In transcription, RNA polymerase ensures that the incoming nucleotide base pairs with the complementary base on the template strand before catalyzing the formation of a phosphodiester bond between the nucleotide and the growing RNA strand. This process ensures that only the correct nucleotide is added to the growing RNA strand.

In Order for a Nucleotide to Be Added to a Growing