A polymerization reaction is made irreversible by:
A) hybridization of a free nucleotide to a template.
B) breaking of the bond between the 2' carbon and the hydroxyl group.
C) creation of a phosphodiester bond.
D) hydrolysis of a pyrophosphate group into two inorganic phosphates.
E) the entry of a free nucleotide into an RNA polymerase.
Correct Answer:
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