A shotgun genome sequencing project is designed in which the average length of a sequenced fragment is 100 nucleotides and each nucleotide is to be sequenced an average of 30 times. For the human genome, which is 3 × 10⁹ base pairs long, how many fragments of 100 nucleotides would need to be sequenced?

Question

Quizplus · Accepted Answer

To calculate the number of 100 nucleotide fragments needed to sequence the human genome (3 × 10^9 base pairs) at 30x coverage, you multiply the genome size by the coverage (30) and then divide by the fragment length (100). This gives (3 × 10^9) × 30 ÷ 100 = 9 × 10^8 fragments.

A Shotgun Genome Sequencing Project Is Designed in Which the Average