Repeated DNA sequences represent a special challenge in genome sequence assembly. Which of the following would be harder to assemble correctly, assuming the number of copies of the repeat can be determined?

Question

Quizplus · Accepted Answer

Sequences containing repeats longer than the DNA fragments to assemble would be harder to assemble correctly. This is because longer repeats create more ambiguous regions in the assembly process, where it is difficult to determine the correct order and orientation of the fragments. Additionally, longer repeats can cause errors in the assembly due to the formation of secondary structures or other complications. Shorter repeats can still pose a challenge, but they are generally easier to resolve and less likely to cause errors.

Repeated DNA Sequences Represent a Special Challenge in Genome Sequence