Given the actions listed below, which are all involved in PCR, select the answer that places them in the CORRECT order. extension
Initiation
Denaturation
Termination
Annealing
A)initiation, extension, termination
B)denaturation, annealing, extension
C)denaturation, extension, termination
D)initiation, denaturation, extension
E)annealing, initiation, termination

Question

Quizplus · Accepted Answer

PCR involves the following steps:
1. Denaturation: The double-stranded DNA is heated to separate it into two single strands.
2. Annealing: The temperature is lowered to allow primers to anneal to the complementary sequence on each DNA strand.
3. Extension: The temperature is raised and Taq polymerase adds nucleotides to the 3' end of the primers, extending the DNA strand.
4. Termination: The reaction is stopped by cooling the sample. In the end, there are now two identical copies of the original double-stranded DNA. So, the correct order is denaturation, annealing, and extension.

Given the Actions Listed Below, Which Are All Involved in PCR