If you were to use CRISPR to cleave DNA at sites flanking a gene, and in this way produce a deletion of the gene, how many CRISPR RNAs would you need?
A)1
B)2
C)3
D)4

Question

Quizplus · Accepted Answer

You would need two CRISPR RNAs to target the sites flanking a gene for deletion. The RNA guide sequences would each bind to one of the flanking sites, allowing the Cas nuclease to cut and create a deletion.

If You Were to Use CRISPR to Cleave DNA at Sites