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A Certain Restriction Enzyme Cleaves Double-Stranded DNA at the Sequence  5’-AAA/TTT-3’  3’-TTT/AAA-5’ \begin{array}{l}\text { 5'-AAA/TTT-3' } \\\text { 3'-TTT/AAA-5' }\end{array}

Question 129

Multiple Choice

A certain restriction enzyme cleaves double-stranded DNA at the sequence shown below, where the slash indicates where each strand is cleaved. Note that the cleavage results in a two-base pair single-stranded region at the 5' end that allows the cleaved ends to undergo base pairing. 5'-AA/ATTT-3'
3'-TTTA/AA-5'
Which of the restriction enzymes, that cleave double-stranded DNA as shown below, would produce overhanging ends able to pair with those produced by the enzyme described above?


A)
 5’-AAA/TTT-3’  3’-TTT/AAA-5’ \begin{array}{l}\text { 5'-AAA/TTT-3' } \\\text { 3'-TTT/AAA-5' }\end{array}

B)
5-AATA/TT-3’  3’-TT/ATAA-5’ \begin{array}{l}5^{\prime} \text {-AATA/TT-3' } \\\text { 3'-TT/ATAA-5' }\end{array}

C)
 5’-GG/ATCC-3’  3’-CCTA/GG-5’ \begin{array}{l}\text { 5'-GG/ATCC-3' } \\\text { 3'-CCTA/GG-5' }\end{array}

D)
5GGA/TCC33CCT/AGG5\begin{array}{l}5^{\prime}-\mathrm{GGA} / \mathrm{TCC}-3^{\prime} \\3^{\prime}-\mathrm{CCT} / \mathrm{AGG}-5 ^{\prime}\end{array}

E)
5GGAT/CC33CCTA/GG5\begin{array}{l}5^{\prime}-\mathrm{GGAT} / \mathrm{CC}-3^{\prime} \\3^{\prime}-\mathrm{CCTA} / \mathrm{GG}-5^{\prime}\end{array}

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