All four dideoxynucleotides can be present in a single Sanger sequencing reaction and still be distinguished because:
A)they are incorporated in the growing chain with different efficiency.
B)they are labeled with different fluorescent dyes.
C)the fragments into which they are incorporated have different mobility in a gel.
D)All of these choices are correct.

Question

Quizplus · Accepted Answer

The dideoxynucleotides are labeled with different fluorescent dyes, allowing them to be distinguished from each other during the sequencing reaction.

All Four Dideoxynucleotides Can Be Present in a Single Sanger