Denaturation of DNA is a necessary step in Southern blotting procedure because it separates double stranded DNA into single stranded DNA.

Question

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Denaturation of DNA involves breaking the hydrogen bonds between the complementary base pairs of the double helix, resulting in two separate single strands of DNA. This is necessary in Southern blotting to allow for the hybridization of a labeled DNA probe to the target DNA sequence.

Denaturation of DNA Is a Necessary Step in Southern Blotting