A Southern blot is a technique that relies on hybridization of:
A)proteins to DNA.
B)a nucleic acid probe to a complementary RNA.
C)a nucleic acid probe to a complementary DNA.
D)None of the other answer options is correct.

Question

Quizplus · Accepted Answer

A Southern blot is a technique used to detect specific DNA fragments in a mixture. It relies on the capability of a nucleic acid (usually DNA) probe to hybridize with a complementary DNA sequence in a sample, which can be separated by gel electrophoresis and transferred to a membrane. The membrane is then incubated with the probe, and the resulting hybridization pattern indicates the presence and size of the target DNA fragment. Therefore, the correct option is C) a nucleic acid probe to a complementary DNA.

A Southern Blot Is a Technique That Relies on Hybridization