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A Graduate Student Wants to Create a Recombinant DNA Molecule \rightarrow

Question 141

Multiple Choice

A graduate student wants to create a recombinant DNA molecule and introduce this molecule into bacteria. What is the CORRECT order of steps that he should follow?


A) choose plasmid/donor DNA \rightarrow cut with restriction enzymes \rightarrow join fragments via DNA ligase \rightarrow transform bacteria
B) choose plasmid \rightarrow cut with restriction enzymes \rightarrow join fragments with DNA ligase \rightarrow introduce donor DNA \rightarrow transform bacteria
C) join plasmid and donor DNA using DNA ligase \rightarrow cut with restriction enzymes \rightarrow transform bacteria
D) transform bacteria \rightarrow choose plasmid/donor DNA \rightarrow join fragments with DNA ligase
E) cut plasmid/donor DNA with restriction enzymes \rightarrow transform bacteria \rightarrow isolate donor DNA/plasmid \rightarrow join fragments with DNA ligase

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