A graduate student wants to create a recombinant DNA molecule and introduce this molecule into bacteria. What is the CORRECT order of steps that he should follow?
A) choose plasmid/donor DNA cut with restriction enzymes join fragments via DNA ligase transform bacteria
B) choose plasmid cut with restriction enzymes join fragments with DNA ligase introduce donor DNA transform bacteria
C) join plasmid and donor DNA using DNA ligase cut with restriction enzymes transform bacteria
D) transform bacteria choose plasmid/donor DNA join fragments with DNA ligase
E) cut plasmid/donor DNA with restriction enzymes transform bacteria isolate donor DNA/plasmid join fragments with DNA ligase
Correct Answer:
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