What feature is shared by restriction fragments produced by the restriction enzymes BclI (T$\downarrow$GATCA) and BglII (A$\downarrow$GATCT)? (The downward arrow denotes the site of cleavage in each strand.)
A)Both fragments have the same 5-base overhang at the 3'-end.
B)Both fragments have the same 5-base overhang at the 5'-end.
C)Both fragments have blunt ends.
D)Both fragments have the same 5-base overhang but one at the 3'-end and the other at the 5'-end.
E)These fragments do not have anything in common.

Question

Quizplus · Accepted Answer

Both BclI and BglII produce restriction fragments with a 5-base overhang, but BclI produces the overhang at the 3'-end (GATC A) while BglII produces the overhang at the 5'-end (A GATCT). Therefore, both fragments have the same overhang, but at opposite ends.

What Feature Is Shared by Restriction Fragments Produced by the Restriction