What is the difference between Sanger sequencing and PCR with regard to the materials needed to perform these reactions?
A)Primers are needed in PCR but not in Sanger sequencing.
B)DNA polymerase is needed in PCR but not in Sanger sequencing.
C)Primers are needed in Sanger sequencing but not in PCR.
D)DNA polymerase is needed in Sanger sequencing but not in PCR.
E)Dideoxynucleotides are needed in Sanger sequencing, but not in PCR.

Question

Quizplus · Accepted Answer

Both Sanger sequencing and PCR require primers and DNA polymerase to initiate and elongate the DNA strand. However, Sanger sequencing uniquely requires dideoxynucleotides (ddNTPs) for chain termination, which are not used in PCR.

What Is the Difference Between Sanger Sequencing and PCR with Regard