The role of dideoxynucleotides in Sanger sequencing is the:
A)removal of specific bases from DNA.
B)termination of DNA synthesis at specific bases.
C)addition of specifically labeled phosphates to the DNA.
D)detection of protons during DNA synthesis.
E)inhibition of restriction enzyme activity.

Question

Quizplus · Accepted Answer

Dideoxynucleotides lack a 3' OH group, which is necessary for DNA polymerase to add the next nucleotide. When a dideoxynucleotide is incorporated into a growing DNA chain, it terminates the chain, resulting in a set of DNA fragments that differ in length by one nucleotide. This allows for the determination of the DNA sequence by size separation and detection of the terminating nucleotide.

The Role of Dideoxynucleotides in Sanger Sequencing Is The