An experiment in DNA editing using CRISPR is carried out in which the editing template DNA contains a G-A mismatch in the region to be edited. At the same nucleotide site in the resulting edited targeted DNA, the base pair is expected to be:
A)G-A.
B)G-C.
C)T-A.
D)C-T.
E)None of the answer options is correct.

Question

Quizplus · Accepted Answer

When a G-A mismatch is present in the editing template DNA, the standard process of DNA repair through base excision repair would lead to the creation of a nick in the DNA strand containing the G-A mismatch. DNA polymerase then repairs the nick by inserting a C, resulting in a C-T base pair in the edited DNA. Therefore, the correct answer is D) C-T.

An Experiment in DNA Editing Using CRISPR Is Carried Out