Which of the following BEST describes the way you would engineer bacterial cells to produce a human protein?
A)Use restriction enzymes to cleave both the donor DNA and the vector DNA.
B)Use restriction enzymes to cleave the donor DNA and to shear the vector DNA into random pieces.
C)Use restriction enzymes to cleave the vector DNA and to shear the donor DNA into random pieces.
D)Randomly shear the donor DNA and the vector DNA.
E)None of the answer options is correct.

Question

Quizplus · Accepted Answer

Using restriction enzymes to cleave both the donor DNA and the vector DNA allows for specific and controlled insertion of the human protein gene into the bacterial genome. This method ensures that the desired gene is inserted into the correct location and minimizes the chances of random insertion or disruption of essential bacterial genes.

Which of the Following BEST Describes the Way You Would