A slightly hazy CSF specimen is diluted 1:10 with acetic acid.A total of 50 cells are counted in five large squares on both sides of the Neubauer counting chamber.The count is reported as:

Question

Quizplus · Accepted Answer

The correct calculation for cell count in this scenario involves multiplying the total cell count by the dilution factor and then dividing by the number of squares counted. Here, 50 cells counted in 5 squares with a 1:10 dilution results in a calculation of (50 cells * 10) / 5 = 100 cells/µL. However, since acetic acid lyses red blood cells and leaves white blood cells intact, the count is specifically of WBCs, and considering both sides of the chamber are counted, the total volume accounted for is doubled, leading to 200 WBCs/µL. The discrepancy in the final answer suggests a misunderstanding in the question's interpretation based on the provided options.

A Slightly Hazy CSF Specimen Is Diluted 1:10 with Acetic