You want to determine which genes are up- and down-regulated in glia after brain injury. Which technique would be best for this type of analysis?
A) RNAseq
B) Immunostaining for a glial specific marker
C) Southern blot
D) In situ hybridization
Anatomical Techniques

Question

Quizplus · Accepted Answer

The best answer is RNAseq as it gives an expression profile of many genes. Immunostaining would just show the glial locations. You have to know specific genes to use Southern blot and in situ hybridization.

You Want to Determine Which Genes Are Up- and Down-Regulated