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An Experiment Reversed Rett Symptoms by Restoring MeCP2 Expression in Young

Question 17

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An experiment reversed Rett symptoms by restoring MeCP2 expression in young mice. Overexpression of MeCP2 causes significant neurological defects so the investigators used an interesting technique where they inserted a transcription stop site flanked by two loxP sites in between the transcription start site and the coding region.
A. What is the advantage of this technique?
B. The investigators then used tamoxifen-inducible CreER excision of the transcription stop site. What was the function of this procedure?

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Using the Cre/lox system provided the ab...

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